Creatinine, Urinary, ELISA Kit
Cat. No. KK135
Store at -20°C
Creatinine is a metabolic waste product formed due to non-enzymatic conversion of creatine and phosphocreatine, the most part of which are found in muscle tissues. Creatinine diffuses into the blood and is excreted by kidneys into the urine. In normal condition, the excretion of creatinine is a relatively constant(1-2% of total creatine pool per day). The amount of creatinine produced is proportional to an individual’s muscle mass. The urinary creatinine levels are commonly used as tool of normalization for other molecules in the urine or other tests. In addition, determination of urinary creatinine is also useful for detecting muscular and kidney disease, and estimation the extent of impairment renal function.
Our kit is convenient to quantify amount of urinary creatinine by using ELISA method.
This kit is only for research use, not for diagnosis.
Highly sensitive and specific
Strip type well, antigen pre-coated microplate
Assay range: 0.625－20mg/dl
The urinary creatinine levels are commonly used as tool of normalization for other molecules in the urine or other tests. In addition, determination of urinary creatinine is also useful for detecting muscular and kidney disease, and estimation the extent of impairment renal function.
This kit is convenient to quantify amount of urinary creatinine by using ELISA method.
- Antigen coated microtiter plate,96 wells 1 plate
- Creatinine standard 250μl × 2
- Antibody diluent 20mL × 1
- HRP- anti Creatinine antibody concentrate(×100) 60μl × 1
- OPD (o-phenylendiamine) tablets 2 tab.
- Substrate solution 30 mL × 1
- Stop solution 15 mL × 1
- Wash buffer concentrate (×20) 30 mL × 1
- Dilution plate 1 plate
Equipments to be supplied by the user
- A microplate reader
- A micropipet
- A microplate washer
- Iyengar MR, Coleman DW, Butler TM. Phosphocreatinine, a high-energy phosphate in muscle, spontaneously forms phosphocreatine and creatinine under physiological conditions. J Biol Chem. 1985 Jun 25;260(12):7562-7.
- Furter R, Kaldis P, Furter-Graves EM, Schnyder T, Eppenberger HM, Wallimann T. Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli. Biochem J. 1992 Dec 15;288 ( Pt 3):771-5.
- Wang ZM, Gallagher D, Nelson ME, Matthews DE, Heymsfield SB. Total-body skeletal muscle mass: evaluation of 24-h urinary creatinine excretion by computerized axial tomography. Am J Clin Nutr. 1996 Jun;63(6):863-9.
- Wyss M, Kaddurah-Daouk R. Creatine and creatinine metabolism. Physiol Rev. 2000 Jul;80(3):1107-213. Review.
- Miller RC, Brindle E, Holman DJ, Shofer J, Klein NA, Soules MR, O'Connor KA. Comparison of specific gravity and creatinine for normalizing urinary reproductive hormone concentrations. Clin Chem. 2004 May;50(5):924-32. Epub 2004 Mar 11.