AteloGene Local Use,Quick Gelation

Product#: 1490
$1,000.00
Availability:
Ships in 1-2 weeks

AteloGene Local Use,Quick Gelation

Cat. No. 1490
Storage 2-10°C
For research use only.



Outline

Improved gel forming speed and transfection efficiency!!
This in vivo transfection kit is designed for delivering siRNA/miRNA into animal tissues, mouse in particular, to introduce the siRNA/miRNA into cells by local administrations.
Atelocollagen is positively charged and can thus electrostatically form complexes with nucleic acids when they are mixed together at appropriate concentrations and ratios. The complexes protect nucleic acids from being degraded by nucleases in in vivo.

What is Atelocollagen?

Collagen is an extracellular matrix found in the dermis, ligaments, bones, etc., and accounts for approximately 30% of the total protein in the human body. The most abundant type of collagen is type I collagen, which has a molecular weight of approximately 300 kDa and comprises three polypeptides. 

The molecular structure of collagen is a right-handed triple helix region and telopeptide (non-helical) regions at the N-terminal and C-terminal of the molecule. These telopeptide regions are composed of two α1 chains and one α2 chain. The triple helix region is conserved among species and shows low immunogenicity, while the telopeptide regions exhibit high immunogenicity. Removal of the telopeptide regions by protease treatment produces atelocollagen, which retains the same properties as collagen. We have developed atelocollagen-based medical devices.

Unwinding of the triple helix of collagen and atelocollagen by heat degeneration produces gelatine. Gelatine is a random coil single polypeptide and has high immunogenicity.
Peptides obtained by hydrolysis with strong acids, strong alkalis, or by enzymatic treatment are called hydrolysed collagen. Gelatine and hydrolysed collagen have totally different properties from collagen due to their structural differences compared to collagen.

1391_fig3.jpg 

Applications

1490_fig1.jpg

Local administration of nucleic acids

Features

  • Prolonged in vivoRNAi effect because atelocollagen, the main component of AteloGene® products, form complexes with siRNA/miRNA
  • Since AteloGene® products are made from atelocollagen, it is nontoxic.
  • AteloGene® Local Use gelates in the body and siRNA/miRNA are slowly released from the gel at the site of administration.
  • AteloGene® Local Use “Quick Gelation” has been modified to increase in vivo transfection efficiency by focusing on faster gel formation at the site of administration.

Kit contents
 

AteloGene® Local Use Quick Gelation prefilled syringe
(Including losses during the preparation steps)

540 μL × 3 syringes

QG buffer

2.5 mL × 1 bottle

2 mL microtube

4 tubes (A spare tube is included)

18G needle

8 needles (2 spare needles are included)

Instruction manual

1 leaflet


Devices and reagents required other than those in the kit

  • 1 mL disposable syringe
  • 27G needle
  • siRNA/miRNA (PAGE- or HPLC-purified grade is recommended.)
  • Container and water for preparation of siRNA/miRNA solution (sterilized, RNase-free)
  • Cooling device (crushed ice, cold block, etc.)
  • Tube rotator (that can tumble and agitate such as TAITEC RT-5, Bibby scientific SB3, etc.)
  • Pipetter and tips (sterilized, RNase-free)
  • High-speed refrigerated centrifuge
  • Anesthetic

Cat. No.

Product

Package

#1492 for Cosmo Bio customers

#1494 for REPROCELL customers

AteloGene® Local Use “QuickGelation”

1 kit (15 administrations) *

*It can be used more than 15 administrations depending on target tissue because one administration is assumed to be 200μl.

Experimental example

Local siRNA administration to mouse xenograft model with AteloGene® Local Use “Quick Gelation”.
1490_fig3.jpg

A remarkable reduction of luciferase expression was observed when a luciferase siRNA (Luc siRNA) was co-administered to dual-luciferase expressing melanoma xenograft mice with AteloGene® Local Use “Quick Gelation”.
 

Preparation of AteloGene® Local Use “Quick Gelation”

Preparation instructions

In this section, the numbers ① – ④ refer to the “Kit contents” on product page. Implement measures to secure an RNase-free environment, as far as possible, to avoid the degradation of siRNA/miRNA in advance.

1)Preparation of AteloGene® Local Use “Quick Gelation” (QG)

Attach the ④18G needle to the ①Prefilled syringe. Eject the whole amount (540 μL) into the ③2 mL microtube. After ejection, cool the microtube containing AteloGene® QG on ice.

An excess amount of AteloGene® QG is provided in the ①Prefilled syringe so that 540 μL of AteloGene® QG can be injected into the ③2.0 mL microtube regardless of losses during the preparation steps.

2) Preparation of siRNA/miRNA solution

Prepare 25 – 50 μM siRNA/miRNA solutions with RNase-free water.

3)Preparation of AteloGene® QG & siRNA/miRNA complex

While cooling on ice, gently pour 540 μL of the ②QG buffer and then 120 μL of the siRNA/miRNA solution onto 540 μL of AteloGene® QG in the ③2.0 mL microtube. Rotate and mix the solutions at 4℃ for 10 minutes. To avoid forming bubbles, the rotation speed should be approximately 12 r.p.m. (in the case of a rotator with a diameter of 20 cm).

Although the mixture may become cloudy during this procedure, it will become a clear solution as the components are thoroughly mixed by rotation.

4)Defoamation of bubbles and preparation for administration


Centrifuge the mixture at 10,000 r.p.m. for 1 minute at 4℃ to defoam bubbles. Attach the ④18G needle to the disposable syringe and slowly draw the mixture while avoiding forming bubbles. Replace the needle with the 27G needle and keep the syringe refrigerated until immediately before administration.

1490_fig2.jpg

References

Local administration in Cancer research

  1. Integrin α6β4-Src-AKT signaling induces cellular senescence by counteracting apoptosis in irradiated tumor cells and tissues. Jung SH, Lee M, Park HA, Lee HC, Kang D, Hwang HJ, Park C, Yu DM, Jung YR, Hong MN, Kim YN, Park HJ, Ko YG, Lee JS. Cell Death Differ. 2018 May 21. [Epub ahead of print] PMID: 29786073
  2. Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1. Nishiyama K, Maruyama R, Niinuma T, Kai M, Kitajima H, Toyota M, Hatanaka Y, Igarashi T, Kobayashi JI, Ogi K, Dehari H, Miyazaki A, Yorozu A, Yamamoto E, Idogawa M4, Sasaki Y, Sugai T, Tokino T, Hiratsuka H, Suzuki H. Cell Death Dis. 2018 Aug 1;9(8):826. PMID: 30069008
  3. Survivin knockdown induces senescence in TTF?1-expressing, KRAS-mutant lung adenocarcinomas. Sumi T, Hirai S, Yamaguchi M, Tanaka Y, Tada M, Yamada G, Hasegawa T, Miyagi Y, Niki T, Watanabe A, Takahashi H, Sakuma Y. Int J Oncol. 2018 Apr 11. [Epub ahead of print] PMID: 29658609
  4. miR-3140 suppresses tumor cell growth by targeting BRD4 via its coding sequence and downregulates the BRD4-NUT fusion oncoprotein. Tonouchi E, Gen Y, Muramatsu T, Hiramoto H, Tanimoto K, Inoue J, Inazawa J. Sci Rep. 2018 Mar 14;8(1):4482. PMID: 29540837
  5. Depleted tumor suppressor miR-107 in plasma relates to tumor progression and is a novel therapeutic target in pancreatic cancer Taisuke Imamura, Shuhei Komatsu, Daisuke Ichikawa, Mahito Miyamae, Wataru Okajima, Takuma Ohashi, Jun Kiuchi, Keiji Nishibeppu, Hirotaka Konishi, Atsushi Shiozaki, Ryo Morimura, Hisashi Ikoma, Toshiya Ochiai, Kazuma Okamoto, Hiroki Taniguchi, Eigo Otsuji Sci Rep. 2017 Jul 18;7(1):5708. PMID: 28720759
  6. Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.
  7. Kato K, Mukudai Y, Motohashi H, Ito C, Kamoshida S, Shimane T, Kondo S, Shirota T. Int J Oncol. 2017 May;50(5):1634-1646. PMID: 28339026
  8. Ephrin-B2 reverse signaling regulates progression and lymph node metastasis of oral squamous cell carcinoma.
  9. Sasabe E, Tomomura A, Tomita R, Sento S, Kitamura N, Yamamoto T. PLoS One. 2017 Nov 30;12(11):e0188965. PMID: 29190834
  10. The microRNA miR-516a-3p regulates the Wnt pathway by targeting extracellular sulfatase 1 in human scirrhous gastric cancers: Anti-metastatic therapy via miRNA-based medicine Yoshifumi Takei, Akiko Suzuki, Keichiro Mihara, Kazuyoshi Yanagihara Medical Research Archives, Vol. 5, Issue 7, July 2017
  11. ANKRD22 promotes progression of non-small cell lung cancer through transcriptional up-regulation of E2F1.Yin J, Fu W, Dai L, Jiang Z, Liao H, Chen W, Pan L, Zhao J. Sci Rep. 2017 Jun 30;7(1):4430. PMID: 28667340
  12. Oncogenic role of rab escort protein 1 through EGFR and STAT3 pathway. Yun UJ, Sung JY, Park SY, Ye SK, Shim J, Lee JS, Hibi M, Bae YK, Kim YN. Cell Death Dis. 2017 Feb 23;8(2):e2621. PMID: 28230863
  13. Pharmacological regulation of bladder cancer by miR-130 family seed-targeting LNA. Egawa H, Jingushi K, Hirono T, Hirose R, Nakatsuji Y, et al. (2016) Integr Mol Med. Volume 3(1): 457-463.
  14. Nek2 siRNA therapy using a portal venous port-catheter system for liver metastasis in pancreatic cancer. Kokuryo T, Hibino S, Suzuki K, Watanabe K, Yokoyama Y, Nagino M, Senga T, Hamaguchi M. Cancer Sci. 2016 Sep;107(9):1315-20. PMID: 27316377 
  15. A therapeutic trial of human melanomas with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3. Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K. Biochim Biophys Acta. 2016 Aug;1860(8):1753-63. PMID: 27068854
  16. Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1α and causes cancer cell death under hypoxia. Oh ET, Kim CW, Kim SJ, Lee JS, Hong SS, Park HJ. Sci Rep. 2016 Jun 6;6:27382. PMID: 27263528
  17. Unfavorable neuroblastoma prognostic factor NLRR2 inhibits cell differentiation by transcriptional induction through JNK pathway. Sheikh A, Takatori A, Hossain MS, Hasan MK, Tagawa M, Nagase H, Nakagawara A. Cancer Sci. 2016 Sep;107(9):1223-32. PMID: 27357360
  18. Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells. Yamane K, Naito H, Wakabayashi T, Yoshida H, Muramatsu F, Iba T, Kidoya H, Takakura N. Sci Rep. 2016 Aug 8;6:30941. PMID: 27499248
  19. Usefulness of microRNA?375 as a prognostic and therapeutic tool in esophageal squamous cell carcinoma.  Isozaki, Y., Hoshino, I., Akutsu, Y., Hanari, N., Mori, M., Nishimori, T., Murakami, K., Akanuma, N., Takeshita, N., Maruyama, T., Toyozumi, T., Takahashi, M., Suito, H., & Matsubara, H. Int J Oncol. 2015 Mar;46(3):1059-66. PMID: 25501018
  20. Arl4c expression in colorectal and lung cancers promotes tumorigenesis and may represent a novel therapeutic target. Fujii S, Matsumoto S, Nojima S, Morii E, Kikuchi A. Oncogene. 2015 Sep 10;34(37):4834-44. PMID: 25486429
  21. MiR-634 activates the mitochondrial apoptosis pathway and enhances chemotherapy-induced cytotoxicity.Fujiwara N, Inoue J, Kawano T, Tanimoto K, Kozaki KI, Inazawa J.Cancer Res. 2015 Sep 15;75(18):3890-901. PMID: 26216549
  22. RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells.Naito A, Yamamoto H, Kagawa Y, Naito Y, Okuzaki D, Otani K, Iwamoto Y, Maeda S, Kikuta J, Nishikawa K, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Ishii H, Doki Y, Mori M, Ishii M.J Biol Chem. 2015 Mar 6;290(10):6326-37. PMID: 25605732
  23. Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction.Ochiya T, Takenaga K, Asagiri M, Nakano K, Satoh H, Watanabe T,Imajoh-Ohmi S, Endo H.Mol Ther Methods Clin Dev. 2015 Apr 1;2:15008. PMID: 26029719
  24. Ubiquilin 2 enhances osteosarcoma progression through resistance to hypoxic stress.Tsukamoto S, Shimada K, Honoki K, Kido A, Akahane M, Tanaka Y, Konishi N.Oncol Rep. 2015 Apr;33(4):1799-806. PMID: 25672654
  25. APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer. Periyasamy M, Patel H, Lai CF, Nguyen VT, Nevedomskaya E, Harrod A, Russell R, Remenyi J, Ochocka AM, Thomas RS, Fuller-Pace F, Gy?rffy B, Caldas C, Navaratnam N, Carroll JS, Zwart W, Coombes RC, Magnani L, Buluwela L, Ali S.Cell Rep. 2015 Oct 6;13(1):108-21. PMID: 26411678
  26. Aurora kinase A has a significant role as a therapeutic target and clinical biomarker in endometrial cancer.Umene K, Yanokura M, Banno K, Irie H, Adachi M, Iida M, Nakamura K, Nogami Y, Masuda K, Kobayashi Y, Tominaga E, oki D.Int J Oncol. 2015 Apr;46(4):1498-506. PMID: 25625960
  27. Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma.Zou XY, Ding D, Zhan N, Liu XM, Pan C, Xia YM.J Invest Dermatol. 2015 Feb;135(2):589-98. PMID: 25184957
  28. RNA interference against cancer/testis genes identifies DUSP21 as a potential therapeutic target in human hepatocellular carcinoma.Deng Q, Li KY, Chen H, Dai JH, Zhai YY, Wang Q, Li N, Wang YP, Han ZG.Hepatology. 2014 Feb;59(2):518-30. PMID: 23929653
  29. MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression.Frampton AE, Castellano L, Colombo T, Giovannetti E, Krell J, Jacob J, Pellegrino L, Roca-Alonso L, Funel N, Gall TM, De Giorgio A, Pinho FG, Fulci V, Britton DJ, Ahmad R, Habib NA, Coombes RC, Harding V, Knösel T, Stebbing J, Jiao LR.Gastroenterology. 2014 Jan;146(1):268-77.e18. PMID: 24120476
  30. Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance. Fukumoto M, Amanuma T, Kuwahara Y, Shimura T, Suzuki M, Mori S, Kumamoto H, Saito Y, Ohkubo Y, Duan Z, Sano K, Oguchi T, Kainuma K, Usami S, Kinoshita K, Lee I, Fukumoto M.Cancer Sci. 2014 Oct;105(10):1351-9. PMID: 25098609
  31. Paired box 2 upregulates androgen receptor gene expression in androgen-independent prostate cancer. Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Hongo F, Kamoi K, Okihara K, Kawauchi A, Miki T.FEBS J. 2014 Oct;281(19):4506-18. PMID: 25132193
  32. High expression of protein tyrosine kinase 7 significantly associates with invasiveness and poor prognosis in intrahepatic cholangiocarcinoma.Jin J, Ryu HS, Lee KB, Jang JJ.PLoS One. 2014 Feb 28;9(2):e90247. PMID: 24587299
  33. Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma.Migita K, Sho M, Shimada K, Yasuda S, Yamato I, Takayama T, Matsumoto S, Wakatsuki K, Hotta K, Tanaka T, Ito M, Konishi N, Nakajima Y.Cancer. 2014 Mar 15;120(6):808-17. PMID: 24249528
  34. Targeting CB2-GPR55 Receptor Heteromers Modulates Cancer Cell Signaling.Moreno E, Andradas C, Medrano M, Caffarel MM, Pérez-Gómez E, Blasco-Benito S, Gómez-Cañas M, Pazos MR, Irving AJ, Lluís C, Canela EI, Fernández-Ruiz J, Guzmán M, McCormick PJ, Sánchez C. J Biol Chem. 2014 Aug 8;289(32):21960-72. PMID: 24942731 Savi F, Forno I, Faversani A, Luciani A, Caldiera S, Gatti S, Foa P, Ricca D, Bulfamante G, Vaira V, Bosari S.
  35. miR-296/Scribble axis is deregulated in human breast cancer and miR-296 restoration reduces tumour growth in vivo.Clin Sci (Lond). 2014 Aug;127(4):233-42. PMID: 24527800
  36. AFAP1L1, a novel associating partner with vinculin, modulates cellular morphology and motility, and promotes the progression of colorectal cancers.Takahashi R, Nagayama S, Furu M, Kajita Y, Jin Y, Kato T, Imoto S, Sakai Y, Toguchida J.Cancer Med. 2014 Aug;3(4):759-74. PMID: 24723436

Local administration in other research
  1. Endogenous Interleukin-1β Is Implicated in Intraplaque Hemorrhage in Apolipoprotein E Gene Null Mice. Suzuki E, Imuta H, Fujita D, Takahashi M, Oba S, Kiyosue A, Nishimatsu H.Circ J. 2018 Mar 23;82(4):1130-1138. PMID: 29467356
  2. Effects of Co-Transfection with Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein on Skeletal Muscle Growth. Od BAYARSAIKHAN, Nobuhiko KAWAI, Hiroyo MORI, Nao KINOUCHI, Takeshi NIKAWA, Eiji TANAKA Journal of Oral Health and Biosciences Vol. 30 (2017) No. 1 p. 1-7 
  3. Targeting of NADPH oxidase in vitro and in vivo suppresses fibroblast activation and experimental skin fibrosis. Dosoki H, Stegemann A, Taha M, Schnittler H, Luger TA, Schröder K, Distler JH, Kerkhoff C, Böhm M. Exp Dermatol. 2017 Jan;26(1):73-81. PMID: 27576129 
  4. Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein β degradation by adenosine receptor A1. Mitani T, Watanabe S, Yoshioka Y, Katayama S, Nakamura S, Ashida H. Biochim Biophys Acta. 2017 Sep 28;1864(12):2438-2448. PMID: 28965824
  5. AAA-ATPase p97 suppresses apoptotic and autophagy-associated cell death in rheumatoid arthritis synovial fibroblasts. Kato M, Ospelt C, Kolling C, Shimizu T, Kono M, Yasuda S, Michel BA, Gay RE, Gay S, Klein K, Atsumi T. Oncotarget. 2016 Sep 27;7(39):64221-64232. PMID: 27623077 
  6. PACAP suppresses dry eye signs by stimulating tear secretion. Nakamachi T, Ohtaki H, Seki T, Yofu S, Kagami N, Hashimoto H, Shintani N, Baba A, Mark L, Lanekoff I, Kiss P, Farkas J, Reglodi D, Shioda S. Nat Commun. 2016 Jun 27;7:12034. PMID: 27345595 
  7. Down-regulation of Fibulin-5 is associated with aortic dilation: role of inflammation and epigenetics. Orriols M, Varona S, Martí-Pàmies I, Galán M, Guadall A, Escudero JR, Martín-Ventura JL, Camacho M, Vila L, Martínez-González J, Rodríguez C. Cardiovasc Res. 2016 Jun 1;110(3):431-42. PMID: 27089918
  8. Tribbles homologue 3 stimulates canonical TGF-β signalling to regulate fibroblast activation and tissue fibrosis.Tomcik M, Palumbo-Zerr K, Zerr P, Sumova B, Avouac J, Dees C, Distler A, Becvar R, Distler O, Schett G, Senolt L, Distler JH.
  9. Ann Rheum Dis. 2016 Mar;75(3):609-16. PMID: 25603829
  10. Inhibition of microRNA-222 expression accelerates bone healing with enhancement of osteogenesis, chondrogenesis, and angiogenesis in a rat refractory fracture model.Yoshizuka M, Nakasa T, Kawanishi Y, Hachisuka S, Furuta T, Miyaki S, Adachi N, Ochi M.J Orthop Sci. 2016 Nov;21(6):852-858. PMID: 27545451
  11. Lentivirus-TAZ Administration Alleviates Osteoporotic Phenotypes in the Femoral Neck of Ovariectomized Rats.Zhang Y, Wang Z, Ding L, Damaolar A, Li Z, Qiu Y, Yin Z.Cell Physiol Biochem. 2016;38(1):283-94. PMID: 26800426
  12. Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells.Chelakkot-Govindalayathil AL, Mifuji-Moroka R, D'Alessandro-Gabazza CN, Toda M, Matsuda Y, Gil-Bernabe P, Roeen Z, Yasuma T, Yano Y, Gabazza EC, Iwasa M, Takei Y.J Thromb Haemost. 2015 Jan;13(1):142-54. PMID: 25399514
  13. Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia.Gomez-Rodriguez V, Orbe J, Martinez-Aguilar E, Rodriguez JA, Fernandez-Alonso L, Serneels J, Bobadilla M, Perez-Ruiz A, Collantes M, Mazzone M, Paramo JA, Roncal C.FASEB J. 2015 Mar;29(3):960-72. PMID: 25414484
  14. RasGRP4 is Aberrantly Expressed in the Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis and Controls their Proliferation.Kono M, Yasuda S, Stevens RL, Koide H, Kurita T, Shimizu Y, Kanetsuka Y, Oku K, Bohgaki T, Amengual O, Horita T, Shimizu T, Majima T, Koike T, Atsumi T.Arthritis Rheumatol. 2015 Feb;67(2):396-407. PMID: 25330932
  15. Female-specific regulation of skeletal muscle mass by USP19 in young mice.Ogawa M, Kitakaze T, Harada N, Yamaji R.J Endocrinol. 2015 Jun;225(3):135-45. PMID: 25901042
  16. Acceleration of healing of the medial collateral ligament of the knee by local administration of synthetic microRNA-210 in a rat model.Muhammad Sakti, Tomoyuki Nakasa, Takeshi Shoji, Muhammad Andry Usman, Yoshitaka Kawanishi, Michio Hamanishi, Irawan Yusuf, Mitsuo Ochi.Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology, Volume 2, Issue 4, October 2015, Pages 129-136.
  17. The effect of administration of double stranded MicroRNA-210 on acceleration of Achilles tendon healing in a rat model.Usman MA, Nakasa T, Shoji T, Kato T, Kawanishi Y, Hamanishi M, Kamei N, Ochi M.J Orthop Sci. 2015 May;20(3):538-46. PMID: 25753838
  18. Attenuated AMH signaling pathway plays an important role in the pathogenesis of ovarian hyperstimulation syndrome.Wang L, Li H, Ai J, Yue J, Li Z, Zhang H, Zhao Y.Am J Transl Res. 2015 Oct 15;7(10):1925-38.
  19. A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice.Ashrin MN, Arakaki R, Yamada A, Kondo T, Kurosawa M, Kudo Y, Watanabe M, Ichikawa T, Hayashi Y, Ishimaru N.J Immunol. 2014 May 1;192(9):4025-31. PMID: 24670797
  20. The CXCR4/SDF1 axis improves muscle regeneration through MMP-10 activity.Bobadilla M, Sáinz N, Abizanda G, Orbe J, Rodríguez JA, Páramo JA, Prósper F, Pérez-Ruiz A.Stem Cells Dev. 2014 Jun 15;23(12):1417-27. PMID: 24548137
  21. Intrathecal miR-96 inhibits Nav1.3 expression and alleviates neuropathic pain in rat following chronic construction injury.Chen HP, Zhou W, Kang LM, Yan H, Zhang L, Xu BH, Cai WH.Neurochem Res. 2014 Jan;39(1):76-83. PMID: 24234845
  22. Inactivation of evenness interrupted (EVI) reduces experimental fibrosis by combined inhibition of canonical and non-canonical Wnt signalling.Distler A, Ziemer C, Beyer C, Lin NY, Chen CW, Palumbo-Zerr K, Dees C, Weidemann A, Distler O, Schett G, Distler JH.Ann Rheum Dis. 2014 Mar 1;73(3):624-7. PMID: 24257024
  23. Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model.Kawanishi Y, Nakasa T, Shoji T, Hamanishi M, Shimizu R, Kamei N, Usman M, Ochi M.Arthritis Res Ther. 2014 Nov 28;16(6):488. PMID: 25430980
  24. Role of Girdin in intimal hyperplasia in vein grafts and efficacy of atelocollagen-mediated application of small interfering RNA for vein graft failure.Miyachi H, Mii S, Enomoto A, Murakumo Y, Kato T, Asai N, Komori K, Takahashi M.
  25. J Vasc Surg. 2014 Aug;60(2):479-489.e5. PMID: 23948670
  26. Osteopontin knockdown in the kidneys of hyperoxaluric rats leads to reduction in renal calcium oxalate crystal deposition.Tsuji H, Shimizu N, Nozawa M, Umekawa T, Yoshimura K, De Velasco MA, Uemura H, Khan SR.Urolithiasis. 2014 Jun;42(3):195-202. PMID: 24619192
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