Anti-Rad51 (human) antibody, chicken polyclonal ( IgY)

Product#: 70-005
$346.00
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Anti-Rad51 (human) antibody, chicken polyclonal ( IgY)


DiagnoCine offers excellent Rad51 I Antibodies and Recombinant proteins  to researchers studying DNA repair, Mitochondrial DNA synthesis, Replication Stress,  Homologous Recombination, and Meiosis.

Human diseases include multiple cancer types  (breast cancer, prostate cancer, renal cancer, and lung cancer & others), aging, congenital mirror movement disorder, and Fanconi anemia.

Rad51 I Antibodies have excellent quality and this highly pure antibody can be adapted for Western blotting, Immunoprecipitation, and Immunofluorescent staining, and other research protocols with optimization.


General information 

Cat. No. 70-005
Size :100 ug
Antigen Species Human
Host Species Chicken
Purification Purified
Reactivity Human, mouse, rat, hamster
Immunogen Recombinant full-size human red51 protein, no tag peptide attached
Application  
  1. Western blotting (1/1,000-1/5,000)
  2. Immunoprecipitation (1/100-1/500)
  3. Immunofluorescent staining (1/100-1/2,000)
Storage Ship at 4℃ and store at -20℃. Do not freeze below -20℃
Form 1 mg/ml in 100 mM NaCl, 20 mM phosphate buffer (pH 7.4), 50% glycerol
Data Link UniProtKB/Swiss-Prot Q06609 (RAD51_HUMAN)

Description

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs, Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogenes and tumor suppressors such as BRACA1, BRACA2, and P53 for the maintenance of genome stability (1). The product was purified (Fig.1) from the eggs laid by immunized chicken with full-size recombinant Rap51 protein expressed in E. coli and purified.

IgY antibody shows significantly less backgrounds than IgG antibody of mammals in immuno-reactions
 
70-005_fig1.png 70-005_fig2.png

Fig.1 SDS-PAGE (without Et-SH) analysis of the purified IgY 

Fig. 2 Western blot analysis of Rad51 in HeLa cell extract The antibody was used at 1/1,000 dilution
 
70-005_fig3.png


Fig. 3. Detection of Rad51 protein foci formation with the anti-Rad51 antibody in X-ray irradiated human fibroblast cells, GM0637.
 
  1. The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for 10 min and washed with PBS three times.
  2. Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times.
  3. Incubated with the anti-Rad51 antibody at 1/100 dilution (left panel) or 1/1,000 dilution (right panel) for 30 min at 37℃ and washed with PBS three times.
  4. As the second antibody, Alexa488-conjugated anti-chicken IgY antibody was used at 1/5,000 dilution.
  5. DNA was stained with Hoechst for 1 min and mounted.

References
This antibody has been used in the following publication.
 
  1. Nakada S et al. RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absence of BRCA1 and 53BP1. Cancer Res. 2012 Oct 1;72(19):4974-83. PMID: 22865450.IF (human, mouse)


Aliases for RAD51 Gene

  • RAD51 Recombinase 2 3 5
  • BRCA1/BRCA2-Containing Complex, Subunit 5 2 3
  • DNA Repair Protein RAD51 Homolog 1 3 4
  • RAD51 Homolog A 3 4
  • HsT16930 2 3
  • HsRad51 2 3
  • HRAD51 3 4
  • RAD51A 3 4
  • BRCC5 2 3
  • FANCR 2 3
  • RECA 3 4
  • RAD51 Homolog (RecA Homolog, E. Coli) (S. Cerevisiae) 2
  • RAD51 (S. Cerevisiae) Homolog (E Coli RecA Homolog) 2
  • RAD51 Homolog (S. Cerevisiae) 2
  • RecA, E. Coli, Homolog Of 3
  • Recombination Protein A 3
  • RecA-Like Protein 3
  • HsRAD51 4
  • MRMV2 3
  • RAD51 5

 
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