Anti-Rad22 (S. pombe) antibody, affinity purified

Product#: 63-003
$375.00
Availability:
Ships in 1-2 weeks

Anti-Rad22 (S. pombe) antibody, affinity purified


General information 

Cat. No. 63-003
Size 50 ug
Antigen Species Yeast 
Host Species Rabbit
Purification Affinity Purified
Label  Unlabeled
Immunogen Recombinant full-size Rad22 protein of S. pombe  Expressed in E. coli.
Purity Affinity-purified with highly purified Rad22 protein  conjugated with agarose beads.
Application    
  1. Western blotting (x 2,000~5,000 dilution)
  2. Immunoprecipitation
  3. Immunofluorescence Staining
  4. ELISA 
Storage Sent at 4℃ or -20℃ and store at -20℃
Form  1 mg/ml in PBS with 50% Glycerol, filter-sterilized
Data Link  SwissProt: P36592 Schizosaccharomyces pombe


Description

Rad22 protein of Schizosaccharomyces pombe (469 aa, 52 kDa) is a functional and structuralhomolog of Saccharomyces cerevisiae and human Rad52 proteins, which play a major role together with Rhp51 in genetic recombination and recombination repair, by mediating strand annealing reaction between homologous DNA strands. 
 
63-003_fig1.png






Fig.1 Western blot analysis of Rad22 in the whole cell extracts with affinity-purified antibody.
M: Molecular weight markers (kD)
Lane 1:Protein size markers (kDa)
Lane 2:Extract of wild-type S. pombe ** indicates Rad22 protein band
63-003_fig2.png







Fig.2. Western blot analysis of Rad22 protein in the whole cell extracts with anti-Rad22 serum before affinity purification.
M: Molecular weight markers (kD)
Lane1: Wild-type strain
Lane2: Rad22 deletion mutant strain 

Entrez Gene 2543086 Schizosaccharomyces pombe 


Reference 

  1. Lehmann AR “Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe.” Mutation Res 363:147-161 Review (1996) PMID: 8765156

Logo_BioAcademia.png

Satisfaction
Quality Rating
Value Rating
Style Rating
X