Anti-Monocyte / Macrophage, Rabbit, Mouse-Mono (RbM2)

Product#: KT015
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Anti-Monocyte / Macrophage, Rabbit, Mouse-Mono (RbM2)

Cat. No. KT015
Storage Store below –20℃, Once thawed, store at 4℃. Repeated freeze-thaw cycles should be avoided

Package Size 50μg (200μL / vial)
Antigen- Rabbit
Host Animal- Mouse
Cross Reactivity- Rabbit
Application- IHC
Unlabeled
Purification- APu 
Clone- RbM2

Class- IgG
Format Mouse monoclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Subclass IgG1
Purification method

The splenic lymphocytes from BALB/c mouse, immunized with thioglycate-elicited rabbit peritoneal macrophages, were fused to myeloma NS-1 cells. The screening of the hybridoma cells was performed on cryostat sections of rabbit spleens. The hybridoma cell line (RbM2) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Working dilution for immunohistochemistry 10μg/mL on frozen sections, not applicable for paraffin sections. 

Description

This anti-rabbit macrophage monoclonal antibody, RbM2, was produced by using thiogllycate-elicited rabbit peritoneal macrophages as immunogen. Immunoelectoron microscopy demonstrates that RbM2 reacts with lysosomes of rabbit macrophages and monocytes. This selective reactivity was confirmed in various experiments by endocytosis. In contrast, dendritic cells, such as follicular dendritic cells (FDCs) of lymphoid follicles, interdigitating cells(IDCs) of lymphoid T zone, or epidermal Langerhan’s cells, are not reactive with this antibody.

KT015_fig1.jpg 
Rabbit Liver (frozen section): Kupffer cells are positively stained. Takeya M., Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan

The antigen recognized by RbM2 is a lysosomal membrane protein with 50,000 molecular weight. Thus, this antibody is very useful for not only in discriminating monocyte / macrophages from various cell populations but also in identifying lysosomes and their related structures in macrophages.

Specificity

Organ

Reaction

Positive

Negative

Thymus

Macrophages in cortex Macrophages in medulla

Epithelial cells Dendritic cells

Spleen

Red pulp macrophages

IDCs in PALS

Marginal zone macrophages FDCs in the germinal centre

Lymph nodes

TB macrophages in follicles Sinus macrophages

IDCs in paracortical areas

FDCs in the germinal center

Lungs

Alveolar macrophages

 

Liver

Kupffer cells

Sinusodal endothelia Perisinusoidal fat-storing cells Parenchymal cells

Skin

Dermal macrophages

Langerhans cells

Epidermal dendritic cells

Brain

Macrophages in the subarachnoid space

Microglial cells

Others

Monocytes Peritoneal macrophages Macrophages in the milky spot of the omentum

Granulocytes Lymphocytes Erythrocytes Polymorphonuclear cells Neuronal cells Muscle cells

PALS=periarteriolar lymphatic sheath

TB=tingible body

IDCs=interdigitating cells

FDCs=follicular dendritic cells


References

  1. Shimokawa Y., Takeya M., Miyauchi Y., Takahashi K. (1990): A monoclonal antibody, RbM2, specific for a lysosomal membrane antigen of rabbit monocyte/macrophages. Immunol. 70: 513-519
  2. Ruan Y., Takahashi K., Naito M. (1995): Immunohistochemical detection of macrophage-derived foam cells and macrophage clony-stimulating factor in pulmonary atherogenesis of cholesterol-fed rabbit. Pathol Int. 45(3): 185-195
  3. Yoshimura N., Arima S., Nakayama M., Sato T., Takahashi K.(1994): Renal impairment and intraglomerular mononuclear phagocytes in choresterol-fed rabbits. Nephron. 68(4): 473-480


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