Anti-EID1 antibody, mouse monoclonal (clone 26)

Product#: 71-185
$375.00
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Anti-EID1 antibody, mouse monoclonal (clone 26)


General Information 
 
Cat. No. 71-185 
Size 100 ug
Antigen Human
Host Species Mouse
Cross Reactivity Human/ Mouse/ Rat
Label Unlabeled 
Clone 26
Immunogen Synthetic peptide containing amino acids 159-187 of human DIE1 protein
Specificity Reacts with human, mouse and rat EID1 proteins
Form  Purified IgG 1mg/ml in PBS (pH 7.4), 50% glycerol, sterilized by filtration. Azide- and carrier-free
Isotype Mouse IgG2a (κ)
Product This antibody was purified by our propriety chromatography under mild conditions as IgG fraction from
    serum-free growth medium of mouse hybridoma clone #26.
Application
  1. Western blotting (~1 ug/ml)
  2. Immunofluorescence staining (1~5 ug/ml)
  3. ELISA Other applications have not been tested.
Storage Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.
Data Link UniProtKB/Swiss-Prot Q9Y6B2 (EID1_HUMAN) 



Description 

EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein, which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation. 

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Fig 1 Identification of the EID1 protein by the monoclonal antibody clone #26 by Western blotting.
Crude cell extracts of MCF7 cells (breast cancer cell line) transfected with control vector pCMV1 (lane 1) or the EID1 expression vector pcDNA3/EID1 (lane 2) were analyzed by Western blotting using anti-EID1 antibody clone #26 as the primary antibody and HRP-conjugated-mouse IgG as the secondary antibody. The EID1 protein was identified as a 21 kDa protein band as shown by an arrow.

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Fig. 2. Immunofluorescent staining of EID1 protein by anti-EID1 antibody (clone 26) in HeLa cells.
EID1 protein is localized in nuclei. Hela cells grown Fixation of the cells in 4% paraformaldehyde over night Permeabilization in 0.25% Triton X-100/PBS for 10 min Blocking in 1.5% BSA/PBS for 30 min Incubation over night in anti-EID1 antibody (clone 2) diluted to 1/1,000 by blocking buffer Incubation for 60min in 2 nd antibody, Goat anti-rabbit IgG conjugated with Alex 488 (1:1,000 dilution) Nuclei were stained with DAPI


References

  1. MacLellan WR et al “A novel Rb- and p300-binding protein inhibits transactivation by MyoD” Mol Cell Biol 20:8903-8915 (2000) PMID: 11073990
  2. Nguyen DX et al “Acetylation regulates the differentiation-specific functions of the retinoblastoma protein” EMBO J 23: 1609-1618 (2004) PMID: 15044952 
 

Aliases for EID1 Gene

  • EP300 Interacting Inhibitor Of Differentiation 1 2 3 5
  • CREBBP/EP300 Inhibitory Protein 1 2 3 4
  • EID-1 2 3 4
  • EP300-Interacting Inhibitor Of Differentiation 1 3 4
  • E1A-Like Inhibitor Of Differentiation 1 3 4
  • 21 KDa PRb-Associated Protein 3 4
  • CREBBP/EP300 Inhibitor 1 2 3
  • C15orf3 3 4
  • RBP21 3 4
  • CRI1 3 4
  • Retinoblastoma Protein-Associated Protein 3
  • Rb- And P300-Binding Protein EID-1 3
  • NB4 Apoptosis Related Protein 3
  • IRO45620 3
  • PNAS-22 3
  • PTD014 3
  • EID1 5
 
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