Anti-ADAM1B Antibody, rat monoclonal (#57)

Product#: 73-010
$450.00
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Anti-ADAM1B Antibody, rat monoclonal (#57)


General information 

Cat. No. 73-010
Size 100 ug
Host Species Rat
Cross Reactivity Mouse    
Reactivity Mouse. Not tested with other species.
Immunogen Mouse sperm
Isotype Rat IgG
Application   Immunocytochemistry (1/100 – 1/300),
    Immunohistochemistry (1/100~1/300), Not suitable for western blotting.
Storage Shipped at 4℃ or at -20℃. Upon arrival, spin-down, aliquot and store at -20℃..
Form Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized.  Azide- and carrier-free.
Data Link UniProtKB Q8R534 (mouse ADAM1b)

Key words

Acrosome reaction, Membrane fusion, Protein trafficking, IZUMO1, Sperm–egg fusion

Function 

May play a role in spermatogenesis, sperm maturation and fertilization..

Molecular mass

89,369 with 806 amino acids. N-terminal signal peptide with 33 amino acids from this protein is processed to give propeptide, which may undergo further processing.
 
 
73-010_fig1Description.png 

Fig.1 Immunofluorescent staining of ADAM1B in sperm fusing with egg by using anti-ADAM1B antibody (#57). Cd9−/− females were mated with Red-IZUMO1 males 12 h after an hCG injection for ovulation induction. Eggs were recovered from the Cd9−/− females 8 h after coitus. Cumulus cells were removed by hyaluronidase treatment. Spermatozoa in the perivitelline space were stained with an Alexa-488-labeled anti-ADAM1B antibody (#57) for 15 min and the eggs were observed using confocal microscopy after being pressed gently between the observation dish and a coverslip. A magnified image of EQ-type sperm from the boxed area in B is shown on the right, C. ADAM1B was stained in green with Alexa-488 conjugated anti-ADAM1B(#57). Red-stained image is Red-IZUMO1.


73-010_fig2Descrition.png 

Fig.2. Immunohistological staining of ADAM1B in mouse testis using anti-ADAM1B antibody (#57). A section of formalin fixed and paraffin embedded mouse testis was treated with the anti-ADAM1B antibody at 1/100 dilution after deparaffization and antigen retrieval. The 2nd antibody, anti-rat IgG conjugated with Alexa Fluor 488 (Abcam) was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merge). The bright field microsopic picture of the same region was shown on the right.
 

Reference 

 
  • Satouh Y., et al (2012) Visualization of the moment of mouse sperm–egg fusion and dynamic localization of IZUMO1. J. Cell Science 125, 4985–4990. PubMed 22946049.
 
Aliases for ADAM1B Gene
 
  • ADAM Metallopeptidase Domain 1B (Pseudogene) 2 3 5
  • ADAM Metallopeptidase Domain 1B, Pseudogene 2
  • ADAM1B 5
 
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