Anti-5-Methylcytosine antibody, mouse IgM

Product#: 51-00
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Anti-5-Methylcytosine antibody, mouse IgM (clone 5MC-CD)


General information 

Cat. No. :51-00_51-003, 51-00_51-004, 51-00_51-005
Size :100 µg (51-00_3), 50 µg (51-00_4, 51-00_5)
Form   51-00_51-003Purified mouse IgM 1 mg/ml in PBS with 50% glycerol, filter-sterilized
51-00_51-004Purified biotinylated IgM, 1 mg/ml in PBS with 50% glycerol, filter-sterilized, azide and carrier free
51-00_51-005Purified FITC-conjugated mouse IgM, 1 mg/ml in PBS with 50% glycerol, filter-sterilized
Immunogen 5-Methylcytosine conjugated to bovine serum albumin
Clone No. 5MC-CD
Host Animal      :Mouse
Isotype :IgM
Reactivity DNA with 5-Methylcytosine (methylated DNA), Any species
Application   Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
Immuno-blotting detection of DNA with 5-methylocytosine on nitrocellulose (Ref. 3 & 4)
   (~1000 fold dilution)
Storage :Shipped at 4℃ or -20℃, and upon arrival, aliquot and store at -20℃.

Description

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.

Figures
51-00_4.png   Fig.1 Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells.
Left: DAPI-stained cells
Middle: Cells stained with anti-5MeC antibody and FITC-conjugated 2nd antibody
Right: Merged image Chloroplast DNA is exclusively methylated in gamete cells.
51-00_5.png   Fig.2 Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the anti-5MeC antibody Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs (For details, see Reference 1).
 
 
Cat. No. Labeled
51-00_51-003 Unlabeled
51-00_51-004 Biotinylated
51-00_51-005 FITC-conjugated

References
 
  1. Sharif J et al “The SRA protein Np95 mediates epigenetic inheritance by recruiting Dmnt1 to methylated DNA” Nature 450: 908-912 (2007) PMID: 17994007
  2. Nishiyama R et al “A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes” PNAS 99: 5925-5930 (2002) PMID: 11983892
  3. Sano H et al “Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine” Biochim Biophys Acta 951:157-65 (1988) PMID: 2847796
  4. Sano H et al “Identification of 5-methycytosine in DNA fragment immobilized on nitrocellulose paper “PNAS 77:3581-3585 (1980) PMID: 6251470
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