Anti Rat Autotaxin/ENPP2 Polyclonal Antibody (N-terminal)

Product#: KM106
$522.57
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Anti Rat Autotaxin/ENPP2 Polyclonal Antibody (N-terminal)



General information 

Cat. No. KM106
Size 25 µg (250 µL / vial)
Host Animal      Rabbit
Format Rabbit polyclonal antibody (0.1mg/mL)
Purification method This antibody was established from the serum of a rabbit immunized with a peptide representing the     
    N-terminal domain of Autotaxin. Purified by peptide affinity chromatography.
Buffer PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Application   Western blotting: 0.5 µg/ml
Shipping and Storage Store below -20℃ Once thawed, store at 4℃. Repeated freeze-thaw cycles should be avoided.
 

Description

Autotaxin, a member of the ENPP (Ectonucleotide pyrophospatase/phosphodiesterase) family of ectoenzymes, was originally identified as a motogen secreted by a melanoma cell line and subsequently reported to promote cell proliferation, cell motility, angiogenesis, and neurite retraction. Autotaxin has lysophospholipase D activity and appears to exert its effects by generation of the lipid mediator lysophosphatidic acid (LPA) from lysophosphatidylcholine and sphingosine 1-phosphate (S1P) from sphingosylphosphorylcholine (SPC) outside the cell. Autotaxin levels are significantly higher in cancerous tissues than in normal tissues, suggesting that this novel extracellular protein may be involved in tumor progression. 

Two Autotaxin antibodies are available;
KM105: Specifically reacts with a C-terminal fragment of Autotaxin
KM106: Specifically reacts with an N-terminal fragment of Autotaxin 

KM106_Description.png Western blotting
Sample
: Conditioned medium from COS7 cells
(1) Autotaxin-trasfected cells
(2) Negative control cells 

Preparation of antibodies and instruction:
Masu M.
Koike S.
University of Tsukuba
Graduate School of Comprehensive Human Sciences


References

  1. Koike S. et al.: The N-terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide. Genes Cells. 2006 Feb;11(2):133-42.
  2. Sato K. et al.: Identification of autotaxin as a neurite retraction-inducing factor of PC12 cells in cerebrospinal fluid and its possible sources. J Neurochem. 2005 Feb;92(4):904-14.
  3. Tanaka M. et al.: Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma. FEBS Lett. 2004 Jul 30;571(1-3):197-204.
  4. Stefan C. et al.: NPP-type ectophosphodiesterases: unity in diversity. Trends Biochem Sci. 2005 Oct;30(10):542-50. 
 

Aliases for ENPP2 Gene

  • Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 2 3 5
  • ATX 2 3 4
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 3 4
  • Extracellular Lysophospholipase D 3 4
  • EC 3.1.4.39 4 51
  • Autotaxin 2 4
  • PD-IALPHA 2 3
  • LysoPLD 3 4
  • E-NPP 2 3 4
  • PDNP2 3 4
  • Phosphodiesterase I/Nucleotide Pyrophosphatase 2 3
  • Plasma Lysophospholipase D 3
  • Autotaxin-T 3
  • AUTOTAXIN 3
  • ATX-X 3
  • ENPP2 5
  • NPP2 3


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