Anti Mouse G5PR Polyclonal Antibody

Product#: KR058
$522.57
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Anti Mouse G5PR Polyclonal Antibody


General information 

Cat. No. KR058
Size 25 µg 
Antigen Species Mouse    
Host Species Rabbit  
Label Unlabeled
Purification method  This antibody was purified from rabbit serum by affinity chromatography.
Application   IP,Western Blot
Working dilution
For immunoprecipitation ; 2 μg , For western blotting ; 1~5 μg/mL 
Storage -20°C
Format Rabbit polyclonal antibody、0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat

Description

G5PR was cloned as novel protein which could bind to Germinal center Associated DNA Primase (GANP). GANP is involved in important events that regulate DNA replication in activated germinal centre B cells. G5PR is homologous to known regulatory B’’ subunits of PP2A, and the G5PR protein is associated with PP5 and PP2A, suggesting that G5PR is a GANP-associated PPase component. G5PR contributes to the regulation of the phosphorylation state of the chromatin-bound or nonbound form of MCM3 (minichromosome replication molecule 3) in vivo. So that, G5PR might regulate MCM3/GANP complex phosphorylation state during cell cycle progression.

This antibody is very useful for immunoprecipitation and westernblotting

 
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Westernblotting

Sample :Myc-GFP-G5PR co-transfected cell lycate
① Immunoprecipitated by anti-G5PR antibody, then ditected by anti-Myc antibody
② Immunoprecipitated by anti-Myc antibody, then ditected by anti-G5PR antibody

Preparation of antibodies and instruction
Drs.Sakaguchi N. and Igarashi H. at Department Immunology,
Kumamoto University School of Medicine 
 

Reference

 

  1. Genes Cells 2002 Aug;7(8):821-34 



Aliases for PPP2R3C Gene

  • Protein Phosphatase 2 Regulatory Subunit B''Gamma 2 3 5
  • Serine/Threonine-Protein Phosphatase 2A Regulatory Subunit B'' Subunit Gamma 3 4
  • Protein Phosphatase 2 (Formerly 2A), Regulatory Subunit B'' 2 3
  • Protein Phosphatase Subunit G5PR 3 4
  • C14orf10 3 4
  • G4-1 2 3
  • G5PR 2 4
  • Protein Phosphatase 2, Regulatory Subunit B'', Gamma 3
  • Rhabdomyosarcoma Antigen MU-RMS-40.6A/6C 4
  • Rhabdomyosarcoma Antigen MU-RMS-40.6A 3
  • Rhabdomyosarcoma Antigen Mu-RMS-40.6C 3
  • Chromosome 14 Open Reading Frame 10 2
  • FLJ20644 2
  • PPP2R3C 5
  • SPGF36 3
  • G5pr 3
  • GDRM 3

 
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