Anti Human L-type Amino Acid Transporter 1 (LAT1) Monoclonal Antibody

Product#: KE023
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Anti Human L-type Amino Acid Transporter 1 (LAT1) Monoclonal Antibody


General information 

Cat. No. :KE023
Size :25 μg (250μL / vial)
Antigen :Human 
Host Animal :Mouse
Cross Reactivity :Human 
Clone :4D9
Class IgM
Format :Mouse monoclonal antibody 0.2mg/ml
Buffer :Block Ace as a stabilizer, containing 0.1% Proclin as bacteriostat
Purification method The spleen cells from mouse, immunized with the synthesized peptide of human C end of LAT-1,
    were fused with mouse P3U1 myeloma cells. The hybridoma cell line with positive reaction was
    grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Peptide affinity
    chromatography.
Storage :Store below -20℃ until needed
Application :Immunohistochemistry: 2-5μg/mL 
    For more detail on the immunohistochemistry, refer to the immuno staining method.
HGNC name* :SLC7A5 (Solute Carrier family 7A5) 

*HGNC: Human Gene Nomenclature Committee

 

Description


Mammalian amino acid transport system is consisted of large variety of transporters, with the reflection of amino acid molecule variety, and is clessfied into various transport systems by the transportative substrate selectivity and the Na+ dependence with the reflection of amino acid molecule variety.

L-type amino acid transporter 1 (LAT1) is a predicted 12 membrane-spanning protein and is unique because it requires an additional single membrane spanning protein, 4F2 heavy chain (4F2hc:CD98), for its functional expression. L-type is Na+ -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, Varine through the plasma membrane.

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LAT1 is, thus transporter responsible for the permeation of aromatic or branched-chain amino acids and amino acid-related drugs such as L-DOPA. LAT has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Highly regulated nature and high level of expression in tumor cell lines, LAT is thought to be up-regulated to support the high protein synthesis for cell glowth and cell activation. This antibody has been proved to be useful for the immunohistochemistry.

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References

  1. Kanai Y.: Curr. Opin. Cell.Biol.9.565-572,1997
  2. Kanai Y., Segawa H., Miyamoto K., Uchino H., Takeda E., Endou H.: Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem.273, 23629-23632,1998
  3. Mastroberardino L, Spindler B., Pfeiffer R., Skelly PJ., Loffing J, Shoemaker CB., Verry F: Amino-acid transport by heterodimers of 4F2hc/CD98 and members of permease family. Nature 395.288-291,1998
  4. Matsuo H., Tsukada S., Nakata T., ChairoungduaA., Kim D. K., Cha S. H. ,Inatomi J., Yorifuji H., Fukuda J., Endou H., Kanai.,:Expression of a system L neutral amino acid transporter at the blood-brain barrie Neuroreport 11 (16),3507-3511,2000
  5. Yanagida O., Kanai Y., Chairoungdua A., Kim D.K., Segawa H., Nii T., Cha S.H., Matsuo H., Fukushima J., Fukasawa Y., Tani Y., Taketani Y., Uchino H., Kim J.Y., Inatomi J., Okayasu I., Miyamoto K., Takeda E., Goya T., and Endou H.:Human L-type amino acid transporter 1 (LAT1): Characterization of function and expression in tumor cell lines. Biochim.Biophys.Acta.1514: 291-302,2001

 

Aliases for SLC7A5 Gene

  • Solute Carrier Family 7 Member 5 2 3 4 5
  • MPE16 2 3 4
  • LAT1 2 3 4
  • E16 2 3 4
  • Solute Carrier Family 7 (Amino Acid Transporter Light Chain, L System), Member 5 2 3
  • Large Neutral Amino Acids Transporter Small Subunit 1 3 4
  • L-Type Amino Acid Transporter 1 3 4
  • Integral Membrane Protein E16 3 4
  • CD98 Light Chain 3 4
  • 4F2 Light Chain 3 4
  • D16S469E 2 3
  • 4F2LC 3 4
  • CD98 2 3
  • Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 3
  • Sodium-Independent Neutral Amino Acid Transporter LAT1 3
  • Y+ System Cationic Amino Acid Transporter 4
  • SLC7A5 5
  • CD98LC 4
  • 4F2 LC 4
  • HLAT1 4

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