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ジェノスタッフ(株)
[Date:December 01 2014]

Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel

Making a section of floating cells or 3D cultured cells (such as embryoid body, spheroid, etc) is burdensome work for researchers.
Conventional methods are cytospin, smear slide, or agarose gel but these method gives damages to your cell sample as the cells are dried or heated during sample preparaiton.
Moreover, for cytospin or smear slide method, you need to start again from sample preparation.

iPGell will greatly help you to achieve this just by using two components.
Cells can be rapidly jellified with unheated condition. Any toxic components affect to cells are not contained.
Jellified sample can be soaked into fixation solution and then you can make a sample block (frozen / paraffin) like the way to make tissue block.

Features

  • Rapid and Easy procedure
    - Just mixing solution A and B, ~2 minutes protocol.

  • Lower damage to Cells (RNA and Protein)
    - as sample is not heated.

  • Various analysis can be done from same sample
    - frozen/paraffin blocks enable you to make a section when you need.

Procedure

Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel

Samples

  • Primary cultured cell
  • Embryoid body
  • Spheroid
  • iPS cells / ES cells
  • Specific cell population sorted by cell sorter

Experiment Examples

  • Morphorogy of mouse derived embryoid body by HE staining

Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel
Embryoid body A (x100) Embryoid body A (x400) Embryoid body B (x100) Embryoid body C (x100)
  • Expression analysis of 3D cultured (spheroid culture) cell

Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel

IHC (Fixed frozen section, x 1000)
Antibody D (Brown) / Counter Stain (Blue)

  • Expression analysis of cultured cell by in situ hybridization

Gene (Blue) / Counter Stain (Pink) Gene (Blue) / Counter Stain (Pink) Gene (Blue) / Counter Stain (Pink)
Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel
Gene E (x400) Gene F (x400) Negative Control (x400)
Supplier Product Code Product Name Size Price
GNSジェノスタッフ
ジェノスタッフ(株)

PG20-1

iPGell

1kit

$500
GNSジェノスタッフ
ジェノスタッフ(株)

PG-trial

iPGell Trial Kit

5tests

$60
Supplier Product Code Product Name Size Price
GNSジェノスタッフ
ジェノスタッフ(株)

PG20-1

iPGell

1kit

$500
Description

iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube., since the gel is formed through non-thermal process and within a few minutes.
The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.

Storage-20°CCAS
LinkiPGel
GNSジェノスタッフ
ジェノスタッフ(株)

PG-trial

iPGell Trial Kit

5tests

$60
Description

* Trial kit is limited to one for each laboratory.

iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube, since the gel is formed through non-thermal process and within a few minutes.
The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.

Storage-20°CCAS
LinkiPGel

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