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(株)ニッピ
[Date:September 08 2014]

Best cell culture substrate for iPS/ES cells iMatrix-511 (Laminin-511 E8)

iMatrix-511 (Laminin-511 E8) is beneficial for your stem cell culture!

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells and it can reduce the risk of the xeno-components contamination in the culture. Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temparature for 3 hours or at 4°C over night.

Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.

Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".
Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion.
Although this is fragment, it has binding ability to alpha6beta1 integrin.
According to Miyazaki's report ( Nat. Commun. 3, 1236 (2012)), Laminin-511 E8 could be used for cell culture of ES cells and iPS cells.

iMatrix-511 (Laminin-511 E8) : Best cell culture substrate for iPS/ES cells!

To date, mouse feeder cells or matrigel have been used for the culture of iPS cells and ES cells.
However, these cell culture substrates have a risk to bring xeno-components in the culture condition so this was said one of the problems for future medical and clinical application.

iMartix can be used for not only stem cells, but various cells, like epidermal cells, vascular endothelial cells.

iMatrix-511 (Laminin-511 E8) : Best cell culture substrate for iPS/ES cells!

Fig. Human Epidemal Cells and Vascular Endothelical Cells cultured on iMartix-511.
More efficient adhesion and extension can be seen when cultured on iMatrix-511.

Features

  • Simple Handling
  • Can be used for various cells - especially iPS / ES cells
  • Reduce the risk of contamination by xeno-components in your culture system.
  • High cell viability and proliferation rate.
  • 175 μg of iMatrix‐511 can coat about 2 to 6 of 6 well plates.

Reference

  • Miner, JH.,et. al., Annu. Rev. Cell Dev. Biol. 20,255~284 (2004).
  • Ido, H.,et. al., J. Biol. Chem. 279,10946~10954 (2004).
  • Ido, H.,et. al., J. Biol. Chem. 282,11144~11154 (2007).
  • Taniguchi, Y.,et. al., J. Biol. Chem. 284,7820~7831 (2009).
  • Miyazaki, T., et. al., Nat. Commun. 3,1236 (2012).
Supplier Product Code Product Name Size Price
NIPニッピ
(株)ニッピ

Datasheet

892001

iMatrix-511

2x175µg

$350
NIPニッピ
(株)ニッピ

892002

iMatrix-511

6x175µg

$900
Supplier Product Code Product Name Size Price
NIPニッピ
(株)ニッピ

892001

iMatrix-511

2x175µg

$350
Description

Recombinant Laminin511-E8 fragments.
iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temperature for 3 hours or at 4C over night.

Storage4°C,Dark StrageCAS
LinkiMatrix-511 (Laminin-511 E8)
NIPニッピ
(株)ニッピ

892002

iMatrix-511

6x175µg

$900
Description

Recombinant Laminin511-E8 fragments.
iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temperature for 3 hours or at 4C over night.

Storage4°C,Dark StrageCAS
LinkiMatrix-511 (Laminin-511 E8)

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