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ノマディックバイオサイエンス(株)
[Date:September 08 2014]

Temperature-responsive Protein A regin for antibody elution Byzen Pro(R)

Very mild and best condition for antibodies
Temparature-responsive Protein A resin "Byzen Pro®"

Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

Byzen Pro(R) is an affinity chromatography resin in which a novel Protein A mutant is used as a ligand.
The product provides a new antibody purification scheme where antibody is eluted under neutral pH by changing temperature, avoiding risk of antibody aggregation and decreased activity at acidic condition.

Features

  • Antibody is eluted under neutral pH. Byzen Pro(R) needs no acidic conditions.
    No risk of aggregation and decreased activity of antibody.
  • Binding and elution are controlled by temperature.
    Antibody is bound below 10ºC and eluted under room temperature condition or lower than 40C.
  • A buffer of your choice can be used for elution.
    PBS buffer can be used for elution step. Buffer exchange is not necessary.
  • Good antibody specificity
    Byzen Pro® is as specific as the conventional Protein A, showing excellent impurity elimination capability.
  • A recovery ratio is 95% or more.

Purification procedure in a closed column

  1. Attach a 5 mL loop before the column, and wash the column with 5 CV of H2O.
  2. Immerse the column and the loop into a 4°C water bath and then equilibrate the column with buffer.
  3. Wait for 5 min for temperature equilibration, and then inject a sample.
  4. After wash the column to remove impurities, stop the pump. Take the column and the loop out of the 4ºC water bath and immerse them into a 40ºC water bath.
  5. Wait for 5 min for temperature equilibration, and then start the pump to elute antibody.
Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

* Purification procedure in open column (no jacket) is also available.

Specificity

  • Human IgG1, IgG2 and IgG4
  • Rabbit IgG and Goat IgG

Specification

  • Bead Structure : Cross-linked polyvinyl alcohol
  • Mean Particle Size : 70 um
  • Max. Back Pressure : 0.3 Mpa,3bar
  • Flow Rate (Recommend) : 125 – 250 cm/h
  • pH Range (Recommend) : pH 6 – pH 8
  • Binding Temperature : 4C
  • Eluting Temperature : 37C – 40C
  • Dynamic Binding Capacity : 26 – 34 mg/ml (20 cm column)
  • Storage Condition : 20% ethanol at 4C

Example data

Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

Human IgG was purified by using Byzen Pro. When CV was 18, temparature has been changed from 4°C to 40°C and collected under neutral pH condition.

Supplier Product Code Product Name Size Price
NOMノマディックバイオサイエンス
ノマディックバイオサイエンス(株)

0809N05

Byzen Pro

5ml

$800
Description

Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity.
In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.

Storage4°CCAS
LinkByzen Pro(R)
NOMノマディックバイオサイエンス
ノマディックバイオサイエンス(株)

0809N25

Byzen ProR

25ml

$2,700
Description

Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity.
In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.

Storage4°CCAS
LinkByzen Pro(R)

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