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フナコシ株式会社
[Date:September 08 2014]

Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring Diffusive Thermoprobe

Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring.
The "Diffusive Thermoprobe" Useful Tools to monitor intracellular temparature

Features

  • The Diffusive Thermoprobe is fluorescent polymeric thermometer.
    It enables us to monitor intracellular temperature near mitochondria, Golgi body etc.
  • Fluorescence lifetime imaging microscopy is required for measuring intracellular temparature following microinjection into cells.
  • The spatial and temperature resolutions of Diffusive Thermoprobe are at the diffraction limited level (200 nm) and 0.18 – 0.5C.
  • Diffusive Thermoprobe is not affected by cellular environmental conditions such as pH, ionic strength, protein etc.

Structure & Functional diagram

Diffusive Thermoprobe : Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring
  • At low temperatures, a thermoresponsive poly-N-n-proplylacrylamide (NNPAM) sequence in Diffusive Thermoprobe assumes an extended structure with hydration of amide linkages, in which a water-sensitive N-{2-[(7-N, N-dimethylaminosulfonyl)-2, 1, 3-benzoxadiazol-4-yl](methyl)amino}ethyl-Nmethylacrylamide (DBD-AA) unit can be quenched by neighbouring water molecules.
  • At higher temperatures, which weaken hydration, the polyNNPAM sequence shrinks because of the hydrophobic interaction among the NNPAM units, resulting in the release of water molecules and strong fluorescence from the DBD-AA unit.
  • An ionic potassium 3-sulfopropyl acrylate (SPA) unit enriches the hydrophilicity of Diffusive Thermoprobe to prevent interpolymeric aggregation within a cell.

Example Data

Diffusive Thermoprobe : Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring

Temperature mapping in living COS7 cells Confocal fluorescence image (left) and fluorescence lifetime image (right) of Diffusive Thermoprobe.

Diffusive Thermoprobe : Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring

Local heat production near the mitochondria in living COS7 cells

  • Temperatures increase near the mitochondria after the inhibition of ATP synthesis by the uncoupler FCCP.
  • Heat production by FCCP resulted in an average temperature increase of 1.02 ± 0.17°C (avg. ± s.d., n = 7) inside COS7 cells 30min after the chemical stimuli.
  • Confocal fluorescence images of Diffusive Thermoprobe (green) and MitoTracker Deep Red FM (red; left) and fluorescence lifetime images of Diffusive Thermoprobe (middle andf right).

Reference

  • Okabe K, et al. Nat Commun. 2012 Feb 28;3:705.
Supplier Product Code Product Name Size Price
FNAフナコシ
フナコシ株式会社

Datasheet

FDV-0002

Diffusive Thermoprobe

100µg

$260
Supplier Product Code Product Name Size Price
FNAフナコシ
フナコシ株式会社

FDV-0002

Diffusive Thermoprobe

100µg

$260
Description

Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy.
This product is licensed by Tokyo University.

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