StemCell Keep is a highly efficient cryopreserving solution by vitrification for
primate ES and iPS cells.

Features
1. Animal-derived Protein-free and DMSO-free formulation. No risk of differentiation effect by DMSO.
2. Maintain ES and iPS cells with colony formation by vitrification.
3. Maintain stem cell pluripotency after thawing.
4. High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies.

• Datasheet

ThelioKeep is a serum-free and dimethyl sulfoxide (DMSO)-free medium for preserving for 1 - 2 weeks at 4℃ .

Features
1. Maintain the morphology and proliferative ability of epithelium and endothelium tissues.
2. Tissues kept in ThelioKeep can be used for various applications.
3. Serum-free and protein-free formulation.

• Datasheet

CryoScarless DMSO-Free is a serum-free and dimethyl sulfoxide (DMSO)-free medium for long-term storage at -80℃ or in liquid nitrogen.

Features
1. Serum-free and DMSO-free formulation.
2. High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins.
3. Consistent and high cell viability after thawing (> 90% for most of the cell lines).

• Datasheet

Make-to-order.
Natural killer (NK) cells can be expanded from human peripheral blood mononuclear cells (PBMCs) without using feeder cells. NK cells can be expanded several hundred to several thousand-fold by 2 - 3 weeks of culture.
One kit is sufficient to expand NK cells from 20 - 50 ml of whole blood. Kit components: NK Cell Initial Flask (75 cm2), NK Cell Initial Medium, NK Cell Initial Cocktail, NK Cell Subculture Medium

make-to-order

Modified ALyS-Basal medium for serum-free culture of human T-lymphocytes and Lymphoblast cells.
Particularly designed for the research of cancer Immuno-therapy.

Modified ALyS-Basal medium for serum-free culture of human T-lymphocytes and Lymphoblast cells.
Particularly designed for the research of cancer Immuno-therapy.

ALyS505N-0 medium containing IL-2(1,000IU/ml).
Particularly designed for the research of Cancer Immuno-therapy by LAK.

Price ; Please inquire to your local distributor or Funakoshi (export@funakoshi.co.jp). Make-to-order.
Natural killer (NK) cells can be expanded from human peripheral blood mononuclear cells (PBMCs) without using feeder cells. NK cells can be expanded several hundred to several thousand-fold by 2 - 3 weeks of culture.
One kit is sufficient to expand NK cells from 20 - 50 ml of whole blood. Kit components: NK Cell Initial Flask (75 cm2), NK Cell Initial Medium, NK Cell Initial Cocktail, NK Cell Subculture Medium

make-to-order

Price ; Please inquire to your local distributor or Funakoshi (export@funakoshi.co.jp). Make-to-order.
Natural killer (NK) cells can be expanded from human peripheral blood mononuclear cells (PBMCs) without using feeder cells. NK cells can be expanded several hundred to several thousand-fold by 2 - 3 weeks of culture.
One kit is sufficient to expand NK cells from 20 - 50 ml of whole blood. Kit components: NK Cell Initial Flask (75 cm2), NK Cell Initial Medium, NK Cell Initial Cocktail, NK Cell Subculture Medium

ALyS505N-0 medium containing IL-2(175IU/ml).
Particularly designed for the research of Cancer Immuno-therapy.

make-to-order

Modified ALyS705 medium for serum-free culture of Hybridoma cells and antibody production.
With human transferring and human recombinant insulin.
Without Albumin.

ALyS505N-0 medium containing IL-2(175IU/ml).
Particularly designed for the research of Cancer Immuno-therapy.

Modified ALyS705 medium for serum-free culture of Hybridoma cells and antibody production.
With human transferring and human recombinant insulin.
Without Albumin.

ALyS505N-0 medium containing IL-2(1,000IU/ml).
Particularly designed for the research of Cancer Immuno-therapy by LAK.

make-to-order

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Gelation temperature : 35.0+/-1.0 ℃
m.p. : 86.0 +/- 1.0 ℃
Gel strength : 700+/-20 g/cm2(1.5% gel)
Gel viscosity : 7.0+/-1.0 cP(1.5% gel, 85 ℃)

Gel Strength : >730+/-20 g/cm2(1.5% gel)
Gel viscosity : 9.0+/-2.0 mPa ・ S(1.5% gel)

Agar for plant tissue culture
Gel Strength : 530+/-20 g/cm2(1.5% gel)

Agar for plant tissue culture
Gel Strength : 630+/-30 g/cm2(1.5% gel)

Gel Strength : >530+/-20 g/cm2(1.5% gel)
Gel viscosity : 8.0+/-2.0 mPa・S(1.5% gel)

Gel Strength : >630+/-20 g/cm2(1.5% gel)
Gel viscosity : 8.0+/-2.0 mPa・S(1.5% gel)

Agar for plant tissue culture
Gel Strength : 730+/-20 g/cm2(1.5% gel)

Gelation temperature : 41.0+/-2.0 ℃
m.p. : 86.0 +/- 2.0 ℃
Gel strength : 650+/-20 g/cm2(1.5% gel)
Gel viscosity : 7.0+/-1.0 Cp(1.5% gel, 85 ℃)

Gel Strength : >630+/-20 g/cm2(1.5% gel)
Gel viscosity : 8.0+/-2.0 mPa・S(1.5% gel)

Gelation temperature : 36.0+/-2.0 ℃
m.p. : 91.0 +/- 2.0 ℃
Gel strength : 800+/-20 g/cm2(1.5% gel)
Gel viscosity : 7.5+/-1.0 cP(1.5% gel, 85 ℃)

Gelation temperature : 36.0+/-2.0 ℃
m.p. : 91.0 +/- 2.0 ℃
Gel strength : 800+/-20 g/cm2(1.5% gel)
Gel viscosity : 7.5+/-1.0 cP(1.5% gel, 85 ℃)

Gel Strength : 650+/-20g/cm2(1.5% gel),
Transition Temp. : 41.0+/-2.0 ℃
m.p. : 86.0+/-2.0 ℃
Ash : <=2.0%,
Water : <=20.0%,
Sulfate : 1.0%,
Ca : <=0.4%,
Fe : <=100ppm

Gelation temperature : 35.0+/-1.0 ℃
m.p. : 86.0 +/- 1.0 ℃
Gel strength : 700+/-20 g/cm2(1.5% gel)
Gel viscosity : 7.0+/-1.0 cP(1.5% gel, 85 ℃)

Recombinant Laminin511-E8 fragments.
iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temperature for 3 hours or at 4C over night.

• Datasheet

Recombinant Laminin511-E8 fragments.
iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Handling is very easy - just dilute in water, coat it in the culture dish or plate and incubate at room temperature for 3 hours or at 4C over night.

Recombinant human insulin solution sterilized and filtered by 0.2μm membrane. 10 mg/ml

Endotoxin :<= 0.1EU
ED50=0.2-0.5 ng/ml (CTLL-2)

Blasticidin S is a peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide bound formation in the ribosomal machinery.
Blasticidin S is used to select transfected cells carrying bsr or BSD resistance genes.

Origin: Streptomyces grissochromogenes
M.W.422.45(free base)

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Poromeric architecture is suitable for growth and differentiation of stem cell.
・Useful for application of float culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)

• Datasheet

Neutral collagen solutionfor tissue culturing,
Eagle's MEM,
pH 7.4
concentration: 0.2 %,
Sterile

• Datasheet

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Good wettability is suitable for growth and differentiation of stem cell.
・Transparent property make enable to observe with a optical microscope.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 96 well microplate.

Neutral collagen solutionfor tissue culturing,
DME medium,
pH 7.4,
concentration: 0.2 %,
Sterile

• Datasheet

Collagen membrane have some features as follows,
Due to membrane transparency, microscopic observation of the cells is possible while culturing.
Study of cell interaction is possible without a co-culture.
Cell culturing on a collagen membrane creates a natural environment that resembles in-vivo systems.
Amino acids and other small molecular weight compounds can move freely though the permeable collagen membrane.

• Datasheet

Size : 100mm × 90mm × 35μm

• Datasheet

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Poromeric architecture is suitable for growth and differentiation of stem cell.
・Useful for application of float culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)

The collagen sponge for cell culturing is a collagen-based device developed for three-dimensional cell culturing. The collagen porous sponge is prepared from a insoluble type I collagen that is derived from bovine Achilles tendon. Cells can penetrate into the sponge and proliferate three-dimensionally. This technique is very useful for tissue engineering studies.

• Datasheet

Acidic collagen solution for tissue culturing, I-AC,
pH 3.0
concentration: 0.3 %
Sterile

• Datasheet

• Datasheet

Acidic collagen solution for tissue culturing,
I-AC
pH 3.0
concentration: 0.5 %,
Sterile

• Datasheet

3mg/mL

• Datasheet

Acidic collagen solution for tissue culturing,
I-PC,
pH 3.0
concentration: 0.3 %,
Sterile

• Datasheet

• Datasheet

Acidic collagen solution for tissue culturing,
I-PC
pH 3.0
concentration: 0.3 %,
Sterile

• Datasheet

• Datasheet

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Poromeric architecture is suitable for growth and differentiation of stem cell.
・Useful for application of float culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)

* Trial kit is limited to one for each laboratory.

iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube, since the gel is formed through non-thermal process and within a few minutes.
The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈 Feature 〉
・Suitable for growth and differentiation of stem cell.
・Provide a suitable environment for 3D culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 96 well microplate.

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Good wettability is suitable for growth and differentiation of stem cell.
・Transparent property make enable to observe with a optical microscope.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 24 well microplate.

Neutral collagen solutionfor tissue culturing,
Hanks's medium
pH 7.4
concentration: 0.2 %,
Sterile

• Datasheet

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈 Feature 〉
・Suitable for growth and differentiation of stem cell.
・Provide a suitable environment for 3D culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 96 well microplate.

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈Feature〉
・Poromeric architecture is suitable for growth and differentiation of stem cell.
・Useful for application of float culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)

• Datasheet

The 'Honeycomb' collagen sponge has a structure in which uniform pores (250-400 μm) are arranged densely in one direction, into which cells can penetrate and proliferate. This structure facilitates the ready supply of nutrients to the cells inside the sponge, and releases metabolic wastes and biochemical products. Cells can proliferate and fill the lumen to form a uniform cell mass.
The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.

• Datasheet

• Datasheet

Collagen sponge for research use is made of highly purified type I Atelocollagen from bovine dermis, and is intended for various research uses including tissue engineering.

• Datasheet

Collagen membrane have some features as follows,
Due to membrane transparency, microscopic observation of the cells is possible while culturing.
Study of cell interaction is possible without a co-culture.
Cell culturing on a collagen membrane creates a natural environment that resembles in-vivo systems.
Amino acids and other small molecular weight compounds can move freely though the permeable collagen membrane.

• Datasheet

Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy.
This product is licensed by Tokyo University.

• Datasheet

Collagen membrane is specially developed from highly purified bovine dermal type I Atelocollagen for single and double layer tissue culture studies.
It is particularly suitable for studying the molecular interactions between two different cell types by culturing on both sides of the membrane. It is potentially useful for the study of artificial organs and in the emerging field of tissue engineering.
The membrane is permeable and allows free passage of amino acids and small molecules, which is important for the absorption and exchange of molecules through the membrane using cell polarity.

• Datasheet

Particulate Thermoprobe is a fluorescent nanogel thermometer for living cells. It aggregates in proportion to increase in the temperature and fluorescence intensity is proportional to its aggregation.
This product is licensed by Tokyo University.

• Datasheet

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈 Feature 〉
・Suitable for growth and differentiation of stem cell.
・Provide a suitable environment for 3D culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 24 well microplate.

GC scaffold is a biodegradable material for the paradigm of stem cell and tissue engineering. It is a PLGA (poly DL-lactic acid glycolic acid (LA : GA = 75 : 25, Mw = 120,000)) based product with validated biocompatibility.

〈 Feature 〉
・Suitable for growth and differentiation of stem cell.
・Provide a suitable environment for 3D culture.
・Easy to isolate of total RNA from cells. (soluble in phenol.)
・Compatible with 24 well microplate.

• Datasheet

The 'Honeycomb' collagen sponge has a structure in which uniform pores (250-400 μm) are arranged densely in one direction, into which cells can penetrate and proliferate. This structure facilitates the ready supply of nutrients to the cells inside the sponge, and releases metabolic wastes and biochemical products. Cells can proliferate and fill the lumen to form a uniform cell mass.
The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.

• Datasheet

iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube., since the gel is formed through non-thermal process and within a few minutes.
The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.

Smear Gell is a reagent kit for attaching cells and making a slide preparation with easy handling with no equipment necessary such as a cytospin centrifuge. After mixing cell suspension and solution on a slide glass, the mixture is made into a form of gel immediately and attached on the slide glass without changing its morphology. This kit is suitable for morphological analysis or immunostaining of cells in culturing, before/after processing cell sorting.
It is easy to male slide preparation from extremely small amount of cells and non-adherent cells.

* Trial kit is limited to one for each laboratory.

Smear Gell is a reagent kit for attaching cells and making a slide preparation with easy handling with no equipment necessary such as a cytospin centrifuge. After mixing cell suspension and solution on a slide glass, the mixture is made into a form of gel immediately and attached on the slide glass without changing its morphology. This kit is suitable for morphological analysis or immunostaining of cells in culturing, before/after processing cell sorting.
It is easy to male slide preparation from extremely small amount of cells and non-adherent cells.